TTF-1 / NKX2.1 (8G7G3/1 + NX2.1/690), CF647 conjugate, 0.1mg/mL

About this product

Recognizes a protein of 40 kDa, identified as Thyroid transcription factor-1 (TTF-1) . TTF-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Nuclei from liver, stomach, pancreas, small intestine, colon, kidney, breast, skin, testes, pituitary, prostate, and adrenal glands are unreactive. Anti-TTF-1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. It can also be used to differentiate small cell lung carcinoma from lymphoid infiltrates.Loss of TTF-1 expression in non-small cell lung carcinoma has been associated with aggressive behavior of such neoplasms. TTF-1 reactivity is also seen in thyroid malignancies. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC470907-100

Size / count

1x 100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

BCH|Benign chorea|BHC|Homeobox protein Nkx2.1|NK2 homeobox 1|NKX2.1|NKX2A|TEBP|Thyroid nuclear factor-1|Thyroid specific enhancer binding protein|Thyroid transcription factor-1 (TTF-1) |Tin man|TITF1|TTF-1

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

NKX2-1

Gene ID

7080

NCBI Gene ID

94367

UniProt

P43699

Swiss Prot

P43699

Host

Mouse

Species Reactivity

Human, Mouse, Rat

Reactivity

NKX2.1|TTF-1

Immunogen

Rat full length TTF-1 recombinant protein (8G7G3/1) ; Recombinant TTF-1 protein (NX2.1/690)

Target Antigen

NKX2.1 | TTF-1

Clonality

Monoclonal

Clone

8G7G3/1 NX2.1/690

Type

Primary

Source

Animal

Origin

Animal

Applications

IHC, FFPE (verified)

Field of Research

Cancer, Transcription factors

Positive Control

MAD109, MLE-15, H441-4, or H345 cells. Normal thyroid or lung.

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

40 kDa

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG' s

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF647