Tyrosinase (T311), CF647 conjugate, 0.1mg/mL

About this product

Recognizes a cluster of proteins between 70-80 kDa, identified as tyrosinase. Occasionally a minor band at 55 kDa is also detected. This MAb shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway; the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this MAb shows tyrosinase in melanotic as well as amelanotic variants. This MAb is a useful marker for melanocytes and melanomas.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC470012-100

Size / count

1x 100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

ATN, CMM8, LB24-AB, Monophenol monooxygenase, OCA1, OCA1A, Oculocutaneous albinism IA, SHEP3, SK29-AB, Tumor rejection antigen AB, TYR

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

TYR

Gene ID

7299

NCBI Gene ID

503555

UniProt

P14679

Swiss Prot

P14679

Host

Mouse

Species Reactivity

Human

Reactivity

Tyrosinase

Immunogen

Recombinant tyrosinase protein

Target Antigen

Tyrosinase

Clonality

Monoclonal

Clone

T311

Type

Primary

Source

Animal

Origin

Animal

Applications

Flow, intracellular (published) |ELISA (published) |IHC, FFPE (verified) |IP (published) |WB (published)

Field of Research

Cancer

Positive Control

SK-MEL-19, SK-MEL-30 cells or Melanoma.

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

70-80 kDa

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. J Clin Pathol (2001) 54:196-200. (IHC, FFPE) PNAS USA (1995) 92: 8125-8129. (WB, IHC, ELISA) J Biol Chem (2001) 276(15): 11933-11938. (WB, IP) Cytometry Part A (2012) 81A: 374381. (Flow, intracellular)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG2a, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF647