ZAP70 (2F3.2), Biotin conjugate, 0.1mg/mL

Catalog number BNCB0811-100

Price for BNCB0811-100

Size / count 1x 100 µL

About this product

ZAP70 is a 70 kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene.In Western blotting of whole cell lysates of normal peripheral blood mononuclear cells, the antibody labels a band corresponding to ZAP70. In Western blotting of whole cell lysates of CD19-positive purified leukemia cells from patients with Ig-unmutated and Ig-mutated CLL, the antibody labels a band corresponding to ZAP70 in the Ig-unmutated CLL samples, whereas no band is observed in the Ig-mutated CLL samples. In Western blotting of cell lysates of Jurkat cells (T-lymphoblastic cell line), the antibody labels a band of 70 kDa protein. In Western blotting of cell lysates of A431 cells (carcinoma cell line), no band is observed. ZAP70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNCB0811-100
Size / count: 1x 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: Selective T cell defect; SRK; STD; Syk-related tyrosine kinase; Tyrosine-protein kinase ZAP-70; TZK; Zeta chain associated protein kinase 70kDa
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: ZAP70
Gene ID: 7535
NCBI Gene ID: 234569
UniProt: P43403
Swiss Prot: P43403
Host: Mouse
Species Reactivity: Human
Reactivity: ZAP70
Immunogen: Recombinant ZAP-70 protein including residues 1-254 and encompassing SH2 domains of human ZAP70
Target Antigen: ZAP70
Clonality: Monoclonal
Clone: 2F3.2
Type: Primary
Source: Animal
Origin: Animal
Applications: Flow, intracellular (verified) |IF (verified) |IHC, FFPE (verified)
Field of Research: Immunology
Positive Control: Jurkat cells. Tonsil or lymph node.
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: 70 kDa
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG2a, kappa
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: Biotin

Frequently Asked Questions

What is the catalog number for ZAP70 (2F3.2), Biotin conjugate, 0.1mg/mL?

The catalog number for ZAP70 (2F3.2), Biotin conjugate, 0.1mg/mL is BNCB0811-100.

What sizes are available for ZAP70 (2F3.2), Biotin conjugate, 0.1mg/mL?

ZAP70 (2F3.2), Biotin conjugate, 0.1mg/mL is available in the following size(s): 1x 100 µL.

Is ZAP70 (2F3.2), Biotin conjugate, 0.1mg/mL in stock?

ZAP70 (2F3.2), Biotin conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures ZAP70 (2F3.2), Biotin conjugate, 0.1mg/mL?

ZAP70 (2F3.2), Biotin conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for ZAP70 (2F3.2), Biotin conjugate, 0.1mg/mL?

Estimated delivery time for ZAP70 (2F3.2), Biotin conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for ZAP70 (2F3.2), Biotin conjugate, 0.1mg/mL?

The safety data sheet for ZAP70 (2F3.2), Biotin conjugate, 0.1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is ZAP70 (2F3.2), Biotin conjugate, 0.1mg/mL used for?

Does this cell line or biologic require a Material Transfer Agreement (MTA)?

Some products, including certain cell lines, plasmids, and tissue cultures, are subject to intellectual property terms and may require an MTA before we can ship. We’ll inform you in advance if this product requires one, and additional handling fees may apply.

What should I do if I need to report a performance issue with a specific lot of this product?

Provide the catalogue number, product name, lot number, and your experimental results to our technical support team. They’ll investigate and, if needed, escalate to the manufacturer, provided the product was used before expiry and stored correctly.

What is the isotype of this antibody?

Isotype information such as IgG1 or IgG2a is listed in the product specifications on the datasheet, relevant for selecting compatible detection reagents.