ZAP70 (2F3.2), CF640R conjugate, 0.1mg/mL

Catalog number BNC400811-100

Price for BNC400811-100

Size / count 1x 100 µL

About this product

ZAP70 is a 70 kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene.In Western blotting of whole cell lysates of normal peripheral blood mononuclear cells, the antibody labels a band corresponding to ZAP70. In Western blotting of whole cell lysates of CD19-positive purified leukemia cells from patients with Ig-unmutated and Ig-mutated CLL, the antibody labels a band corresponding to ZAP70 in the Ig-unmutated CLL samples, whereas no band is observed in the Ig-mutated CLL samples. In Western blotting of cell lysates of Jurkat cells (T-lymphoblastic cell line), the antibody labels a band of 70 kDa protein. In Western blotting of cell lysates of A431 cells (carcinoma cell line), no band is observed. ZAP70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNC400811-100
Size / count: 1x 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: Selective T cell defect; SRK; STD; Syk-related tyrosine kinase; Tyrosine-protein kinase ZAP-70; TZK; Zeta chain associated protein kinase 70kDa
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: ZAP70
Gene ID: 7535
NCBI Gene ID: 234569
UniProt: P43403
Swiss Prot: P43403
Host: Mouse
Species Reactivity: Human
Reactivity: ZAP70
Immunogen: Recombinant ZAP-70 protein including residues 1-254 and encompassing SH2 domains of human ZAP70
Target Antigen: ZAP70
Clonality: Monoclonal
Clone: 2F3.2
Type: Primary
Source: Animal
Origin: Animal
Applications: Flow, intracellular (verified) |IF (verified) |IHC, FFPE (verified)
Field of Research: Immunology
Positive Control: Jurkat cells. Tonsil or lymph node.
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: 70 kDa
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Notes: Protect from light
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG2a, kappa
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: CF640R

Frequently Asked Questions

What is the catalog number for ZAP70 (2F3.2), CF640R conjugate, 0.1mg/mL?

The catalog number for ZAP70 (2F3.2), CF640R conjugate, 0.1mg/mL is BNC400811-100.

What sizes are available for ZAP70 (2F3.2), CF640R conjugate, 0.1mg/mL?

ZAP70 (2F3.2), CF640R conjugate, 0.1mg/mL is available in the following size(s): 1x 100 µL.

Is ZAP70 (2F3.2), CF640R conjugate, 0.1mg/mL in stock?

ZAP70 (2F3.2), CF640R conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures ZAP70 (2F3.2), CF640R conjugate, 0.1mg/mL?

ZAP70 (2F3.2), CF640R conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for ZAP70 (2F3.2), CF640R conjugate, 0.1mg/mL?

Estimated delivery time for ZAP70 (2F3.2), CF640R conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for ZAP70 (2F3.2), CF640R conjugate, 0.1mg/mL?

The safety data sheet for ZAP70 (2F3.2), CF640R conjugate, 0.1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is ZAP70 (2F3.2), CF640R conjugate, 0.1mg/mL used for?

What is the molecular weight of the target protein?

Molecular weight information, where applicable, is listed on the datasheet under target details, useful for confirming the correct band on western blot.

How long will it take to receive this product if it’s custom made?

Lead times depend on the product type: custom polyclonal antibodies typically take 12-14 weeks, monoclonal antibodies 15-17 weeks, made-to-order recombinant proteins 4-8 weeks, and made-to-order recombinant antibodies 6-8 weeks. These are estimates and may shift due to production delays.

What is the recommended dilution for this antibody?

A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.