CD3e (T-Cell Marker) (UCHT1), CF740 conjugate, 0.1mg/mL

About this product

Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR) . Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC742473-100

Size / count

1x 100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

CD3 epsilon; CD3 TCR complex; T cell antigen receptor complex epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T3E; TCRE; TiT3 complex

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

CD3E

Gene ID

916

NCBI Gene ID

3003

UniProt

P07766

Swiss Prot

P07766

Host

Mouse

Species Reactivity

Chimpanzee, Human

Reactivity

CD3e

Immunogen

Human infant thymocytes and peripheral blood lymphocytes from a Sezary Syndrome donor

Target Antigen

CD3e

Clonality

Monoclonal

Clone

UCHT1

Type

Primary

Source

Animal

Origin

Animal

Applications

Functional studies (published) |ELISA (published) |Flow, surface (verified) |IF (verified) |IP (published) |WB (published)

Positive Control

Human peripheral blood. Jurkat cells. Tonsil or lymph node.

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% rBSA, 0.05% azide

Molecular Weight

20 kDa

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. _x000D_ 1. Blood (1988) 71(3): 603-612. (immunofluorescence, western)_x000D_ 2. J Immunol Meth (1988) 106(1): 127-133. (ELISA)_x000D_ 3. J Exp Med (1991) 173: 7-17. (immunoprecipitation)_x000D_ 4. J Immunol (2002) 168: 3235-3241. (functional studies)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Species

Mus musculus (mouse), Bos taurus (bovine)

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF740