CD73 (Immuno-Oncology Target) (NT5E/2503), CF740 conjugate, 0.1mg/mL

Catalog number BNC742503-100

Price for BNC742503-100

Size / count 1x 100 µL

About this product

CD73 (also designated ecto-5'-nucleotidase, E5NT, NT, NT5, NTE, eN and eNT) is a glycosyl-phosphatidylinositol (GPI) -anchored adhesion protein that catalyzes the dephosphorylation of extracellular purine and pyrimidine nucleotides to their corresponding bioactive nucleosides. CD73 is a dimer of two identical subunits that depends on GPI to link with the external face of the plasma membrane. Similar to other GPI-anchored proteins, CD73 mediates co-stimulatory signals in T cell activation. CD73 has few structural variants, yet elicits diverse biological function through differential regulation in endothelial cells (EC), subpopulations of B and T cells, germinal center follicular dendritic cells and on thymic medullary reticular fibroblasts. For example, IgG mediated neutralization of CD73 interferes with lymphocyte adhesion to EC, and blocks aggregation of germinal center B cells and follicular dendritic cells. Furthermore, IgG-mediated targeting of lymphocyte CD73, but not of endothelial cell CD73, causes shedding of CD73 and tyrosine phosphorylation of proteins. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNC742503-100
Size / count: 1x 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: 5' NT; 5' nucleotidase (CD73) ; 5' nucleotidase precursor; 5' nucleotidase; ecto; 5' nucleotidase; ecto (CD73) ; 5'-NT; 5'-nucleotidase; 5NTD_HUMAN; CD73; CD73 antigen; E5NT; Ecto 5' nucleotidase; Ecto-5'-nucleotidase; eN; eNT; NT; NT5; NT5E; NTE; Purine 5 Prime Nucleotidase
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: NT5E
Gene ID: 4907
NCBI Gene ID: 153952
UniProt: P21589
Swiss Prot: P21589
Host: Mouse
Species Reactivity: Human
Reactivity: CD73
Immunogen: Recombinant full length human NT5E protein
Target Antigen: CD73
Clonality: Monoclonal
Clone: NT5E/2503
Type: Primary
Source: Animal
Origin: Animal
Applications: Flow (verified) |IF (verified) |IHC, FFPE (verified) |WB (verified)
Positive Control: Tonsil, Prostate or Lung Carcinoma.
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% rBSA, 0.05% azide
Molecular Weight: 71 kDa
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Notes: Protect from light
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG1, kappa
Species: Mus musculus (mouse), Bos taurus (bovine)
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: CF740

Frequently Asked Questions

What is the catalog number for CD73 (Immuno-Oncology Target) (NT5E/2503), CF740 conjugate, 0.1mg/mL?

The catalog number for CD73 (Immuno-Oncology Target) (NT5E/2503), CF740 conjugate, 0.1mg/mL is BNC742503-100.

What sizes are available for CD73 (Immuno-Oncology Target) (NT5E/2503), CF740 conjugate, 0.1mg/mL?

CD73 (Immuno-Oncology Target) (NT5E/2503), CF740 conjugate, 0.1mg/mL is available in the following size(s): 1x 100 µL.

Is CD73 (Immuno-Oncology Target) (NT5E/2503), CF740 conjugate, 0.1mg/mL in stock?

CD73 (Immuno-Oncology Target) (NT5E/2503), CF740 conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures CD73 (Immuno-Oncology Target) (NT5E/2503), CF740 conjugate, 0.1mg/mL?

CD73 (Immuno-Oncology Target) (NT5E/2503), CF740 conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for CD73 (Immuno-Oncology Target) (NT5E/2503), CF740 conjugate, 0.1mg/mL?

Estimated delivery time for CD73 (Immuno-Oncology Target) (NT5E/2503), CF740 conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for CD73 (Immuno-Oncology Target) (NT5E/2503), CF740 conjugate, 0.1mg/mL?

The safety data sheet for CD73 (Immuno-Oncology Target) (NT5E/2503), CF740 conjugate, 0.1mg/mL is available here: https://cdn.gentaur.com/products/37/68dd0296061270361e0ffebb/msds/bnc742503-100.pdf.

What is CD73 (Immuno-Oncology Target) (NT5E/2503), CF740 conjugate, 0.1mg/mL used for?

What is the recommended dilution for this antibody?

A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

Can this product be used in multiplex assays?

Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.