CD81 / TAPA-1 (1.3.3.22), CF647 conjugate, 0.1mg/mL

About this product

This antibody recognizes a protein of 26 kDa, identified as CD81 (Workshop VI; Code CD81.1) . CD81 has a very broad cellular distribution, being expressed on T- and B-lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells. Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive. CD81 is a member of a family of tetraspanin transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82. It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC470391-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

CD81; Target of the antiproliferative antibody 1 (TAPA-1) ; Tetraspanin-28; Tspan-28

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

CD81

Gene ID

975

NCBI Gene ID

54457

UniProt

P60033

Swiss Prot

P60033

Host

Mouse

Species Reactivity

Human, Rat

Reactivity

CD81|TAPA-1

Immunogen

B-Cell line derived from a Burkitt lymphoma

Target Antigen

CD81 | TAPA-1

Clonality

Monoclonal

Clone

1.3.3.22

Type

Primary

Source

Animal

Origin

Animal

Applications

Functional studies (published) |Exosome staining (verified) |Flow, surface (verified) |IF (verified) |IHC, FFPE (verified) |WB (published)

Field of Research

Exosomes/EVs, Immunology

Positive Control

Ramos, Daudi, Raji, U266, HUT-78, HUT-102, Jurkat, HL-60, KG1a, A293, A431, MG63, MCF-7, and human lymphocytes. Tonsil or liver tissue.

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

26 kDa

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. Hepatology (2008) 48(6): 1761. (functional studies) Microbiol Immunol (2004) 48(5): 417–426. (western)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF647