EMI1 (EMI1/1176), 0.2mg/mL

About this product

This antibody recognizes a 56 kDa protein, which is identified as Early Mitotic Inhibitor-1 (EMI1) . It regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC) . Emi1 is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb) . Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNUB1176-100

Size / count

1x 100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

Early Mitotic Inhibitor-1 (EMI1) ; FBX5; Fbxo31

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

FBXO5

Gene ID

26271

NCBI Gene ID

520506

UniProt

Q9UKT4

Swiss Prot

Q9UKT4

Host

Mouse

Species Reactivity

Human

Reactivity

EMI1

Immunogen

Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein

Target Antigen

EMI1

Clonality

Monoclonal

Clone

EMI1/1176

Type

Primary

Source

Animal

Origin

Animal

Applications

IHC, FFPE (verified) |WB (verified)

Field of Research

Cell cycle

Positive Control

HeLa, 293 or HepG2 cells. Ovarian carcinoma.

Stability

2 years

Concentration

0.2 mg/mL

Buffer

PBS, 0.05% BSA, 0.05% azide

Molecular Weight

56 kDa

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG2a, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

PBS, 0.05% BSA, 0.05% sodium azide (CAS 26628-22-8)

Conjugate

Purified, with BSA