Anti-GFP Tag (Mouse Monoclonal)

About this product

Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.

Specifications

Catalog number

G096

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Immunogen

GFP from the jellyfish Aequorea victoria N-Terminal peptide-KLH conjugates (first 20 aa).

Clonality

Monoclonal

Clone

LGB-1

Type

Antibodies-Tag

Applications

WB,ELISA,Other

Concentration

100ug/100ul

Purity

Affinity purified

Buffer

PBS, pH 7.4 with 0.05% sodium azide.

Shipping Conditions

Ice Pack

Storage Conditions

This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Fragment

IgG1

Specificity

Recognizes native and denatured forms of GFP and its variants EGFP, YFP, EYFP, and CFP.