Anti-Abeta (1-40/42) | 4D8 | oligomer specific monoclonal targeting amyloid-beta

About this product

Alzheimer's disease is a progressive neurodegenerative disorder characterized by neurofibrillary tangles and depositionof plaques containing the amyloid-ß (Aß) peptide. Aß is derived through proteolytic cleavage of the amyloid precursor protein resulting in various peptide lengths corresponding to 38-43 amino acids. Aß1-42 is particularly prone to self-aggregate and its aggregates are strongly linked to development of the disease. During in vitro Aß self-assembly, highly cytotoxic metastable intermediates (oligomers) are generated ranging from 8 kDa to over 100 kDa. Several reports suggest a similar situation in vivo. We have generated a monoclonal antibody, specific for Aß oligomers, by immunizing mice with partly aggregated Aß1-40 peptide. The antibody 4D8 is a conformational specific antibody that specifically can detect oligomeric structures of Aß (1-42). The reactivity is strongly enhanced within an oligomeric range of approximately 4-15 Aß monomers while it shows only very low affinity towards mature fibrils and soluble monomers.

Specifications

Catalog number

Y030015

Size / count

25 µg

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Reactivity

Human

Immunogen

Partly aggregated, recombinant peptide corresponding to the human Abeta (1-40). Amino acid sequence: D-A-E-F-R-H-D-S-G-Y-E-V-H-H-Q-K-L-V-F-F-A-E-D-V-G-S-N-K-G-A-I-I-G-L-M-V-G-G-V-V

Clonality

Monoclonal

Type

Antibodies-Monoclonal

Applications

IHC,ELISA

Concentration

0.5ug/1ul

Purity

Affinity purified

Buffer

PBS, pH 7.4 with 0.02% sodium azide.

Shipping Conditions

Ice Pack

Storage Conditions

This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Fragment

IgM

Specificity

No confirmed exceptions from predicted reactivity known in the moment

Applications Notes

Coating antibody at 2 µg/ml (ELISA), 1: 500 (IHC)