DyLight 649 Conjugated Affinity Purified anti-Rabbit IgG [H&L] [Goat]

About this product

Minimum Cross Reactivity to Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins

Specifications

Catalog number

SD005

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Reactivity

Rabbit

Immunogen

Rabbit IgG, whole molecule

Type

Antibodies-Secondary

Applications

This is designed for immunofluorescence microscopy, fluorescence based plate assays (ELISA) and fluorescent western blotting. This is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Concentration

1.0 mg/ml (by UV absorbance at 280 nm)

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins. This will react with heavy chains of rabbit IgG and with light chains of most rabbit immunoglobulins.

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Shipping Conditions

Ice Pack

Storage Conditions

This product is stable for several weeks at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. Expiration date is one (1) year from date of receipt if properly stored.

Applications Notes

FLUORESCENT WESTERN BLOT >1:20,000 ELISA >1:10,000 IMMUNOFLUORESCENT MICROSCOPY >1:5,000 OTHER APPLICATIONS User Optimized