Phospho-Histone H3 (T3) Recombinant Rabbit Monoclonal Antibody [JE42-48]

About this product

Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Specifications

Catalog number

HA721796

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

HIST1 cluster, H3J antibody Histone gene cluster 1, H3 histone family, member E antibody Histone gene cluster 1, H3G antibody H3 histone family, member J antibody HIST1 cluster, H3E antibody HIST1 cluster, H3I antibody Histone gene cluster 1, H3C antibody FLJ92264 antibody H3 histone family, member A antibody H3 histone family, member B antibody H3 histone family, member C antibody H3 histone family, member D antibody H3 histone family, member F antibody H3 histone family, member H antibody H3 histone family, member I antibody H3 histone family, member K antibody H3 histone family, member L antibody H3 histone family, member T antibody H3 histone, family 3A antibody H3.1 antibody H3.3A antibody H3/a antibody H3/b antibody H3/c antibody H3/d antibody h3/f antibody H3/h antibody H3/i antibody H3/j antibody H3/k antibody H3/l antibody H3/t antibody H31_HUMAN antibody H3F1K antibody H3F3 antibody H3F3A antibody H3FA antibody H3FB antibody H3FC antibody H3FD antibody H3FF antibody H3FH antibody H3FI antibody H3FJ antibody H3FK antibody H3FL antibody HIST1 cluster, H3A antibody HIST1 cluster, H3B antibody HIST1 cluster, H3C antibody HIST1 cluster, H3D antibody HIST1 cluster, H3F antibody HIST1 cluster, H3G antibody HIST1 cluster, H3H antibody HIST1H3A antibody HIST1H3B antibody HIST1H3C antibody HIST1H3D antibody HIST1H3E antibody HIST1H3F antibody HIST1H3G antibody HIST1H3H antibody HIST1H3I antibody HIST1H3J antibody HIST3H3 antibody Histone 1, H3a antibody Histone 1, H3b antibody Histone 1, H3c antibody Histone 1, H3d antibody Histone 1, H3e antibody Histone 1, H3f antibody Histone 1, H3g antibody Histone 1, H3h antibody Histone 1, H3i antibody Histone 1, H3j antibody histone 3, H3 antibody histone cluster 1 H3 family member a antibody histone cluster 1 H3 family member b antibody histone cluster 1 H3 family member c antibody histone cluster 1 H3 family member d antibody histone cluster 1 H3 family member e antibody histone cluster 1 H3 family member f antibody histone cluster 1 H3 family member g antibody histone cluster 1 H3 family member h antibody histone cluster 1 H3 family member i antibody histone cluster 1 H3 family member j antibody Histone cluster 1, H3a antibody Histone cluster 1, H3b antibody Histone cluster 1, H3c antibody Histone cluster 1, H3d antibody Histone cluster 1, H3e antibody Histone cluster 1, H3f antibody Histone cluster 1, H3g antibody Histone cluster 1, H3i antibody Histone cluster 1, H3j antibody Histone gene cluster 1, H3 histone family, member A antibody Histone gene cluster 1, H3 histone family, member B antibody Histone gene cluster 1, H3 histone family, member C antibody Histone gene cluster 1, H3 histone family, member D antibody Histone gene cluster 1, H3 histone family, member F antibody Histone gene cluster 1, H3 histone family, member G antibody Histone gene cluster 1, H3 histone family, member H antibody Histone gene cluster 1, H3 histone family, member I antibody Histone gene cluster 1, H3 histone family, member J antibody Histone gene cluster 1, H3A antibody Histone gene cluster 1, H3B antibody Histone gene cluster 1, H3D antibody Histone gene cluster 1, H3E antibody Histone gene cluster 1, H3F antibody Histone gene cluster 1, H3H antibody Histone gene cluster 1, H3I antibody Histone gene cluster 1, H3J antibody Histone H 3 antibody Histone H3.1 antibody histone H3.1t antibody Histone H3.2 antibody Histone H3/a antibody Histone H3/b antibody Histone H3/c antibody Histone H3/d antibody Histone H3/f antibody Histone H3/h antibody Histone H3/i antibody Histone H3/j antibody Histone H3/k antibody Histone H3/l antibody Histone H3/m antibody Histone H3/o antibody

Abbreviation

H3-3A, H3-3B

Swiss Prot

P68431 Human, P68433 Mouse, Q6LED0 Rat

Cellular Locus

Nucleus, Chromosome.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Thr3 of Human Histone H3.

Isotype

IgG

Clone

JE42-48

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P

Positive Control

HeLa treated with 20% FBS and 100nM Calyculin A for 30 minutes cell lysate, NIH/3T3 treated with 20% PBS and 100nM Calyculin A for 30 minutes cell lysate, mouse breast tissue, mouse skin tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IHC-P: 1:200-1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 15 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes