Netrin 1 Recombinant Rabbit Monoclonal Antibody [JE38-87]

About this product

Netrin-1 is a protein that in humans is encoded by the NTN1 gene. Netrin is included in a family of laminin-related secreted proteins. The function of this gene has not yet been defined; however, netrin is thought to be involved in axon guidance and cell migration during development. Mutations and loss of expression of netrin suggest that variation in netrin may be involved in cancer development. NTN1 has been shown to interact with Deleted in Colorectal Cancer. During the development of the central nervous system, when the dorsal and ventral signaling is being established, the floor plate is an important site for crossing for groups of neural processes at the dorsal midline. Once crossed through the floor plate, these groups are now referred to as commissural axons. These neuronal cell bodies are signaled by Netrin 1 to be attracted to the floor plate from the dorsal half of the neural tube. NTN1 is a gene that encodes for the protein, Netrin-1. In a study done in knockout mice with a depletion of floor plate Netrin-1, it was shown that corticospinal axon tract midline crossing was disrupted. This study was done to show characteristics of patients with human congenital mirror movement disorder. Afterwards, proper positioning of axons and midline crossing are pioneered by the Slit-Robo system where Slit proteins act as axonal repellents and Robo proteins (Robo-1, Robo-2, Robo-3) act in conjunction with Slit ligands to be their receptors. Slit with Robo-1 and Robo-2 repel axonal extension at the midline thereby commissural axons expressing Robo-3 cross the midline by interfering with Slit/Robo-1, Robo-2 repulsive activity. After crossing the floor plate, Robo-3 is downregulated and Slit/Robo-1, Robo-2 continue to express their repulsiveness at the midline. Furthermore, mutations in Robo-3 cause horizontal gaze palsy with progressive scoliosis.

Specifications

Catalog number

HA721545

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

epididymis tissue protein Li 131P antibody NET1_HUMAN antibody Netrin 1, mouse, homolog of antibody Netrin 1-like antibody Netrin-1 antibody Netrin1 antibody Ntn 1 antibody NTN 1L antibody NTN1 antibody NTN1L antibody Unc6 antibody

Abbreviation

Netrin-1 Epididymis tissue protein Li 131P NTN1 NTN1L

Swiss Prot

O95631 Human, O09118 Mouse, Q924Z9 Rat

Cellular Locus

Secreted > extracellular space > extracellular matrix.

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant protein within Human Netrin 1 aa 505-604 / 604.

Isotype

IgG

Clone

JE38-87

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB

Positive Control

Human small intestine tissue lysate, mouse kidney tissue lysate, mouse heart tissue lysate.

Concentration

1 mg/mL

Dilution

WB: 1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 68 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes