RPB3 Recombinant Rabbit Monoclonal Antibody [JE34-81]

About this product

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft. Component of the RNA polymerase II (Pol II) core complex consisting of 12 subunits: a ten-subunit catalytic core composed of POLR2A/RPB1, POLR2B/RPB2, POLR2C/RPB3, POLR2I/RPB9, POLR2J/RPB11, POLR2E/RPABC1, POLR2F/RPABC2, POLR2H/RPABC3, POLR2K/RPABC4 and POLR2L/RPABC5 and a mobile stalk composed of two subunits POLR2D/RPB4 and POLR2G/RPB7, protruding from the core and functioning primarily in transcription initiation. Part of Pol II (G) complex, in which Pol II core associates with an additional subunit POLR2M; unlike conventional Pol II, Pol II (G) functions as a transcriptional repressor. Part of TBP-based Pol II pre-initiation complex (PIC), in which Pol II core assembles with general transcription factors and other specific initiation factors including GTF2E1, GTF2E2, GTF2F1, GTF2F2, TCEA1, ERCC2, ERCC3, GTF2H2, GTF2H3, GTF2H4, GTF2H5, GTF2A1, GTF2A2, GTF2B and TBP; this large multi-subunit PIC complex mediates DNA unwinding and targets Pol II core to the transcription start site where the first phosphodiester bond forms.

Specifications

Catalog number

HA722448

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

DNA directed RNA polymerase II 33 kDa polypeptide antibody DNA directed RNA polymerase II subunit C antibody DNA directed RNA polymerase II subunit RPB3 antibody DNA-directed RNA polymerase II 33 kDa polypeptide antibody DNA-directed RNA polymerase II subunit C antibody DNA-directed RNA polymerase II subunit RPB3 antibody hRPB33 antibody hsRPB3 antibody POLR 2C antibody POLR2 C antibody Polr2c antibody Polymerase (RNA) II (DNA directed) polypeptide C, 33kDa antibody polymerase RNA II DNA directed polypeptide C 33kDa antibody RNA polymerase II polypeptide C antibody RNA polymerase II subunit 3 antibody RNA polymerase II subunit B3 antibody RPB 3 antibody RPB3 antibody RPB3_HUMAN antibody RPB31 antibody RPB33 antibody

Abbreviation

DNA-directed RNA polymerase II subunit RPB3 RNA polymerase II subunit 3; RNA polymerase II subunit B3 DNA-directed RNA polymerase II 33 kDa polypeptide (RPB33) DNA-directed RNA polymerase II subunit C RPB31 POLR2C A-152E5.7

Swiss Prot

P19387 Human, P97760 Mouse,

Cellular Locus

Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat, Monkey

Immunogen

Recombinant protein within Human RPB3 aa 1-150 / 275.

Isotype

IgG

Clone

JE34-81

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB

Positive Control

SiHa cell lysate, HepG2 cell lysate, HeLa cell lysate, COS-1 cell lysate, mouse kidney tissue lysate, rat kidney tissue lysate.

Concentration

1 mg/mL

Dilution

WB: 1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 31 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes