PAI1 Mouse Monoclonal Antibody [A9G8]

About this product

Plasminogen activator inhibitor-1 (PAI-1) also known as endothelial plasminogen activator inhibitor or serpin E1 is a protein that in humans is encoded by the SERPINE1 gene. Elevated PAI-1 is a risk factor for thrombosis and atherosclerosis. PAI-1 is a serine protease inhibitor (serpin) that functions as the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), the activators of plasminogen and hence fibrinolysis (the physiological breakdown of blood clots). It is a serine protease inhibitor (serpin) protein (SERPINE1). The other PAI, plasminogen activator inhibitor-2 (PAI-2) is secreted by the placenta and only present in significant amounts during pregnancy. In addition, protease nexin acts as an inhibitor of tPA and urokinase. PAI-1, however, is the main inhibitor of the plasminogen activators. PAI-1's main function entails the inhibition of urokinase plasminogen activator (uPA), an enzyme responsible for the cleavage of plasminogen to form plasmin. Plasmin mediates the degradation of the extracellular matrix either by itself or in conjunction with matrix metalloproteinases. In this scenario, PAI-1 inhibits uPA via active site binding, preventing the formation of plasmin. Additional inhibition is mediated by PAI-1 binding to the uPA/uPA receptor complex, resulting in the latter's degradation. Thus, PAI can be said to inhibit the serine proteases tPA and uPA/urokinase, and hence is an inhibitor of fibrinolysis, the physiological process that degrades blood clots. In addition, PAI-1 inhibits the activity of matrix metalloproteinases, which play a crucial role in invasion of malignant cells through the basal lamina. PAI-1 is mainly produced by the endothelium (cells lining blood vessels), but is also secreted by other tissue types, such as adipose tissue.

Specifications

Catalog number

HA601152

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

Clade E antibody Endothelial plasminogen activator inhibitor antibody Nexin antibody Nexin plasminogen activator inhibitor type 1 antibody PAI 1 antibody PAI antibody PAI-1 antibody PAI1_HUMAN antibody PLANH1 antibody Plasminogen activator inhibitor 1 antibody Plasminogen activator inhibitor type 1 antibody Serine (or cysteine) proteinase inhibitor antibody Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody Serine proteinase inhibitor clade E member 1 antibody serpin antibody Serpin E1 antibody Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody Serpin peptidase inhibitor clade E antibody Serpine 1 antibody SERPINE1 antibody

Abbreviation

SERPINE1, PAI1, PLANH1

Swiss Prot

P05121 Human, P22777 Mouse, P20961 Rat

Cellular Locus

Secreted.

Host

Mouse

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within human PAI1 aa 31-80 / 402.

Isotype

IgG1

Clone

A9G8

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

WB, IHC-P, IF-Tissue, FC

Positive Control

No heat NIH/3T3 cell lysate, no heat NIH/3T3 serum starved for 4 hours then treated with 10ng/mL TGF-β for 21 hours add 300ng/mL BFA for 18 hours cell lysate, NIH/3T3 cell lysate, NIH/3T3 serum starved for 4 hours then treated with 10ng/mL TGF-β for 21 hours add 300ng/mL BFA for 18 hours cell lysate, HUVEC cell lysate, mouse placenta tissue lysate, rat placenta tissue lysate, human breast carcinoma tissue, human placenta tissue, HepG2, NIH/3T3.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IHC-P: 1:5,000 ;IF-Tissue: 1:1,000 ;FC: 1:500-1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 45 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No