PU.1 (SPI-1) (B-Cell Marker) (PU1/2146), 0.2mg/mL — image 1 of 7

Product details

PU.1 (SPI-1) (B-Cell Marker) (PU1/2146), 0.2mg/mL

Catalog number BNUB2146-100

Price for BNUB2146-100

Size / count 1x 100 µL

About this product

PU.1 is a member of the ETS family of transcription factors and is important for normal B-cell development. It is expressed in the myeloid lineage and in immature as well as mature B-lymphocytes, with the exception of plasma cells. PU.1 is expressed in germinal center B-cells and mantle B-cells. Various lymphomas are also positive for this marker. It is essential during early B-cell differentiation. The absence of PU.1 results in total block of B-cell development at the pre-pro stage. PU.1 plays a key role in normal myeloid differentiation, and regulates the expression of immunoglobulin and other genes that are important for B-cell development. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNUB2146-100
Size / count: 1x 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: Transcription Factor spi1; 31kDa Transforming Protein; Hematopoietic Transcription Factor PU.1; SFPI1; SPI1; SPIA; Spleen focus forming virus (SFFV) proviral integration oncogene spi1
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: SPI1
Gene ID: 6688
NCBI Gene ID: 502511
UniProt: P17947
Swiss Prot: P17947
Host: Mouse
Species Reactivity: Human
Reactivity: PU.1
Immunogen: Recombinant fragment (around aa 16-170) of human PU.1 protein (Exact sequence is proprietary)
Target Antigen: PU.1
Clonality: Monoclonal
Clone: PU1/2146
Type: Primary
Source: Animal
Origin: Animal
Applications: Flow, intracellular (verified) |IF (verified) |IHC, FFPE (verified) |WB (verified)
Field of Research: Immunology, Transcription factors
Positive Control: K-562 Cells, THP-1 cells. Lymph Node, Spleen, Hodgkin's Lymphoma, Colon Carcinoma.
Stability: 2 years
Concentration: 0.2 mg/mL
Buffer: PBS, 0.05% BSA, 0.05% azide
Molecular Weight: 40 kDa
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG2b, kappa
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: PBS, 0.05% BSA, 0.05% sodium azide (CAS 26628-22-8)
Conjugate: Purified, with BSA

Frequently Asked Questions

What is the catalog number for PU.1 (SPI-1) (B-Cell Marker) (PU1/2146), 0.2mg/mL?

The catalog number for PU.1 (SPI-1) (B-Cell Marker) (PU1/2146), 0.2mg/mL is BNUB2146-100.

What sizes are available for PU.1 (SPI-1) (B-Cell Marker) (PU1/2146), 0.2mg/mL?

PU.1 (SPI-1) (B-Cell Marker) (PU1/2146), 0.2mg/mL is available in the following size(s): 1x 100 µL.

Is PU.1 (SPI-1) (B-Cell Marker) (PU1/2146), 0.2mg/mL in stock?

PU.1 (SPI-1) (B-Cell Marker) (PU1/2146), 0.2mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures PU.1 (SPI-1) (B-Cell Marker) (PU1/2146), 0.2mg/mL?

PU.1 (SPI-1) (B-Cell Marker) (PU1/2146), 0.2mg/mL is manufactured by Biotium.

What is the estimated delivery time for PU.1 (SPI-1) (B-Cell Marker) (PU1/2146), 0.2mg/mL?

Estimated delivery time for PU.1 (SPI-1) (B-Cell Marker) (PU1/2146), 0.2mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for PU.1 (SPI-1) (B-Cell Marker) (PU1/2146), 0.2mg/mL?

The safety data sheet for PU.1 (SPI-1) (B-Cell Marker) (PU1/2146), 0.2mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is PU.1 (SPI-1) (B-Cell Marker) (PU1/2146), 0.2mg/mL used for?

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.