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Product details

CD86 (Dendritic Cells Maturation Marker) (rC86/1146), 0.2mg/mL

Catalog number BNUB2083-500

Price for BNUB2083-500

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Size / count 1x 500 µL

About this product

This antibody recognizes a protein of 70 kDa, which is identified as CD86. CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin's disease.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number
BNUB2083-500
Size / count
1x 500 µL
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9007-83-4
Synonyms
Activation B7-2 antigen; B lymphocyte activation antigen B72; CD28 antigen ligand 2; CD28LG2; CLS1; CTLA-4 counter-receptor B7.2; Early T-cell co-stimulatory molecule 1; ETC-1; FUN-1; LAB72; Ly-58; MB7-2; TS/A-2
UNSPSC
41116161
UNSPSC Description
Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name
CD86
Gene ID
942
NCBI Gene ID
171182
UniProt
P42081
Swiss Prot
P42081
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Reactivity
CD86
Immunogen
Recombinant full-length human CD86 protein
Target Antigen
CD86
Clonality
Recombinant Monoclonal
Clone
RC86/1146
Type
Primary
Source
Animal
Origin
Animal
Applications
Flow (verified) |IF (verified) |IHC, FFPE (verified)
Field of Research
Cancer immunotherapy/immune checkpoint, Immunology
Positive Control
293T or Jukat cells. Monocytes and dendritic cells, activated T, B and natural killer cells in lymph node or tonsil.
Stability
2 years
Concentration
0.2 mg/mL
Buffer
PBS, 0.05% BSA, 0.05% azide
Molecular Weight
70 kDa
Shipping Conditions
RT (ND)
Storage Conditions
Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life
Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment
IgG1, kappa
Species
Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification
PBS, 0.05% BSA, 0.05% sodium azide (CAS 26628-22-8)
Conjugate
Purified, with BSA

Frequently Asked Questions

What is the catalog number for CD86 (Dendritic Cells Maturation Marker) (rC86/1146), 0.2mg/mL?

The catalog number for CD86 (Dendritic Cells Maturation Marker) (rC86/1146), 0.2mg/mL is BNUB2083-500.

What sizes are available for CD86 (Dendritic Cells Maturation Marker) (rC86/1146), 0.2mg/mL?

CD86 (Dendritic Cells Maturation Marker) (rC86/1146), 0.2mg/mL is available in the following size(s): 1x 500 µL.

Is CD86 (Dendritic Cells Maturation Marker) (rC86/1146), 0.2mg/mL in stock?

CD86 (Dendritic Cells Maturation Marker) (rC86/1146), 0.2mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures CD86 (Dendritic Cells Maturation Marker) (rC86/1146), 0.2mg/mL?

CD86 (Dendritic Cells Maturation Marker) (rC86/1146), 0.2mg/mL is manufactured by Biotium.

What is the estimated delivery time for CD86 (Dendritic Cells Maturation Marker) (rC86/1146), 0.2mg/mL?

Estimated delivery time for CD86 (Dendritic Cells Maturation Marker) (rC86/1146), 0.2mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for CD86 (Dendritic Cells Maturation Marker) (rC86/1146), 0.2mg/mL?

The safety data sheet for CD86 (Dendritic Cells Maturation Marker) (rC86/1146), 0.2mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is CD86 (Dendritic Cells Maturation Marker) (rC86/1146), 0.2mg/mL used for?

This antibody recognizes a protein of 70 kDa, which is identified as CD86. CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density

What is the molecular weight of the target protein?

Molecular weight information, where applicable, is listed on the datasheet under target details, useful for confirming the correct band on western blot.

How long will it take to receive this product if it’s custom made?

Lead times depend on the product type: custom polyclonal antibodies typically take 12-14 weeks, monoclonal antibodies 15-17 weeks, made-to-order recombinant proteins 4-8 weeks, and made-to-order recombinant antibodies 6-8 weeks. These are estimates and may shift due to production delays.

What is the recommended dilution for this antibody?

A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.

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