Cytokeratin 10 (KRT10) (Suprabasal Epithelial Marker) (KRT10/1990R), Biotin conjugate, 0.1mg/mL

Catalog number BNCB1990-500

Price for BNCB1990-500

Size / count 1x 500 µL

About this product

This MAb recognizes a protein of 56.5 kDa, identified as cytokeratin 10 (CK10) . CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNCB1990-500
Size / count: 1x 500 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: BCIE; BIE; EHK; Keratin Type I Cytoskeletal 10; KRT10
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: KRT10
Gene ID: 3858
NCBI Gene ID: 99936
UniProt: P13645
Swiss Prot: P13645
Host: Rabbit
Species Reactivity: Human, Mouse
Reactivity: Cytokeratin 10|KRT10
Immunogen: Recombinant human full-length KRT10 protein
Target Antigen: Cytokeratin 10 | KRT10
Clonality: Recombinant Monoclonal
Clone: KRT10/1990R
Type: Primary
Source: Animal
Origin: Animal
Applications: IHC, FFPE (verified)
Field of Research: Cancer, Cytoskeleton
Positive Control: A431, HeLa, MCF7 cells. Esophagus or tonsil.
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: 56.5 kDa
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG
Species: Oryctolagus cuniculus (domestic rabbit), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: Biotin

Frequently Asked Questions

What is the catalog number for Cytokeratin 10 (KRT10) (Suprabasal Epithelial Marker) (KRT10/1990R), Biotin conjugate, 0.1mg/mL?

The catalog number for Cytokeratin 10 (KRT10) (Suprabasal Epithelial Marker) (KRT10/1990R), Biotin conjugate, 0.1mg/mL is BNCB1990-500.

What sizes are available for Cytokeratin 10 (KRT10) (Suprabasal Epithelial Marker) (KRT10/1990R), Biotin conjugate, 0.1mg/mL?

Cytokeratin 10 (KRT10) (Suprabasal Epithelial Marker) (KRT10/1990R), Biotin conjugate, 0.1mg/mL is available in the following size(s): 1x 500 µL.

Is Cytokeratin 10 (KRT10) (Suprabasal Epithelial Marker) (KRT10/1990R), Biotin conjugate, 0.1mg/mL in stock?

Cytokeratin 10 (KRT10) (Suprabasal Epithelial Marker) (KRT10/1990R), Biotin conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Cytokeratin 10 (KRT10) (Suprabasal Epithelial Marker) (KRT10/1990R), Biotin conjugate, 0.1mg/mL?

Cytokeratin 10 (KRT10) (Suprabasal Epithelial Marker) (KRT10/1990R), Biotin conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for Cytokeratin 10 (KRT10) (Suprabasal Epithelial Marker) (KRT10/1990R), Biotin conjugate, 0.1mg/mL?

Estimated delivery time for Cytokeratin 10 (KRT10) (Suprabasal Epithelial Marker) (KRT10/1990R), Biotin conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Cytokeratin 10 (KRT10) (Suprabasal Epithelial Marker) (KRT10/1990R), Biotin conjugate, 0.1mg/mL?

The safety data sheet for Cytokeratin 10 (KRT10) (Suprabasal Epithelial Marker) (KRT10/1990R), Biotin conjugate, 0.1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is Cytokeratin 10 (KRT10) (Suprabasal Epithelial Marker) (KRT10/1990R), Biotin conjugate, 0.1mg/mL used for?

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.