Mouse/Rat TNF alpha Recombinant Rabbit Monoclonal Antibody [PSH03-29] - BSA and Azide free (Capture)

About this product

Tumor necrosis factor alpha (TNF-alpha ), also known as cachectin and TNFSF1A, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism. TNF-alpha is also involved in a number of pathological conditions including asthma, Crohn's disease, rheumatoid arthritis, neuropathic pain, obesity, type 2 diabetes, septic shock, autoimmunity, and cancer. Mouse TNF-alpha is synthesized as a 26 kDa type II transmembrane protein that consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 179 aa extracellular domain (ECD). Within the ECD, mouse TNF-alpha shares 95% aa identity with rat, and 80% aa identity with canine, equine, feline, human, rabbit, and porcine TNF-alpha. It is produced by a wide variety of immune, epithelial, endothelial, and tumor cells. TNF-alpha is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface. Cell surface TNF-alpha can both induce the lysis of tumor cells and virus infected cells, and generate its own downstream cell signaling following ligation by soluble TNF RI. Shedding of membrane bound TNF-alpha by TACE/ADAM17 releases the bioactive cytokine, a 55 kDa soluble trimer containing the TNF-alpha extracellular domain. TNF-alpha binds the ubiquitous 55-60 kDa TNF RI and the hematopoietic cell-restricted 78-80 kDa TNF RII, both of which are also expressed as homotrimers. Both type I and type II receptors bind TNF-alpha with comparable affinity and can promote NFkB activation. Only TNF RI, however, contains a cytoplasmic death domain which triggers the activation of apoptosis. Soluble forms of both types of receptors are released into human serum and urine, and can neutralize the biological activity of TNF.

Specifications

Catalog number

HA721974

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

APC1 APC1 protein Cachectin DIF Differentiation inducing factor Macrophage cytotoxic factor Tnf TNF superfamily member 2 TNF superfamily, member 2 TNF, macrophage derived TNF, monocyte derived TNF-a TNF-alpha TNFA TNFA_HUMAN TNFSF2 Tumor necrosis factor Tumor necrosis factor (TNF superfamily member 2) Tumor necrosis factor alpha Tumor necrosis factor ligand superfamily member 2 Tumor Necrosis Factor, Membrane Form Tumor necrosis factor, soluble form

Abbreviation

Tumor necrosis factor Cachectin TNF-alpha Tumor necrosis factor ligand superfamily member 2 (TNF-a) Tumor necrosis factor, membrane form Alternative names: N-terminal fragment (NTF) Intracellular domain 1 (ICD1) Intracellular domain 2 (ICD2) C-domain 1 C-domain 2 Tumor necrosis factor, soluble form Tnf Tnfa, Tnfsf2

Swiss Prot

P06804 Mouse, P16599 Rat

Cellular Locus

Secreted.

Host

Rabbit

Species Reactivity

Mouse, Rat

Immunogen

Recombinant protein within Rat TNF alpha aa 80-235 (P16599).

Isotype

IgG

Clone

PSH03-29

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

ELISA (Cap)

Positive Control

Recombinant rat TNF alpha protein, Recombinant mouse TNF alpha protein.

Concentration

1 mg/mL

Dilution

ELISA (Cap): Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH03-30] to Mouse/Rat TNF alpha (Detector) (HA721975) and recombinant standard Mouse TNF alpha (HA210527) and Rat TNF alpha (HA210538) as the standard. The reference range value is 12.3-3000 pg/ml.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4).

Molecular Weight

Predicted band size: 25.8 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes