RNA Polymerase II (CTD4H8), CF740 conjugate, 0.1mg/mL

Catalog number BNC742929-100

Price for BNC742929-100

Size / count 1x 100 µL

About this product

RNA polymerase II (Pol II) is an enzyme that is composed of 12 subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1, 840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic activity and, together with the second largest subunit, forms the active center of the Pol II enzyme. Additionally, the large subunit participates in forming the DNA-binding domain of Pol II, a groove that is necessary for transcription of the DNA template. Without proper function of the large subunit, mRNA synthesis and subsequent transcription elongation cannot occur. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNC742929-100
Size / count: 1x 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: DNA-directed RNA polymerase II largest subunit RNA polymerase II 220kd subunit; DNA-directed RNA polymerase II subunit RPB1; hRPB220; hsRPB1; POLR2; Polr2a; POLRA; Polymerase (RNA) II (DNA directed) polypeptide A 220kDa; RNA polymerase II subunit B1; RPBh1; RpIILS; RPO2; RPOL2
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: POLR2A
Gene ID: 5430
NCBI Gene ID: 270017
UniProt: P24928
Swiss Prot: P24928
Host: Mouse
Species Reactivity: Human, Mouse, Rat
Reactivity: RNA Polymerase II
Immunogen: Ten repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser5
Target Antigen: RNA Polymerase II
Clonality: Monoclonal
Clone: CTD4H8
Type: Primary
Source: Animal
Origin: Animal
Applications: IHC, FFPE (verified) |WB (verified)
Positive Control: HAP1, K562, PC3, HePG2, NIH3T3 cells. Human testis.
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% rBSA, 0.05% azide
Molecular Weight: 192-253 kDa
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Notes: Protect from light
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG1, kappa
Species: Mus musculus (mouse), Bos taurus (bovine)
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: CF740

Frequently Asked Questions

What is the catalog number for RNA Polymerase II (CTD4H8), CF740 conjugate, 0.1mg/mL?

The catalog number for RNA Polymerase II (CTD4H8), CF740 conjugate, 0.1mg/mL is BNC742929-100.

What sizes are available for RNA Polymerase II (CTD4H8), CF740 conjugate, 0.1mg/mL?

RNA Polymerase II (CTD4H8), CF740 conjugate, 0.1mg/mL is available in the following size(s): 1x 100 µL.

Is RNA Polymerase II (CTD4H8), CF740 conjugate, 0.1mg/mL in stock?

RNA Polymerase II (CTD4H8), CF740 conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures RNA Polymerase II (CTD4H8), CF740 conjugate, 0.1mg/mL?

RNA Polymerase II (CTD4H8), CF740 conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for RNA Polymerase II (CTD4H8), CF740 conjugate, 0.1mg/mL?

Estimated delivery time for RNA Polymerase II (CTD4H8), CF740 conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for RNA Polymerase II (CTD4H8), CF740 conjugate, 0.1mg/mL?

The safety data sheet for RNA Polymerase II (CTD4H8), CF740 conjugate, 0.1mg/mL is available here: https://cdn.gentaur.com/products/37/68dd029b061270361e101d60/msds/bnc742929-100.pdf.

What is RNA Polymerase II (CTD4H8), CF740 conjugate, 0.1mg/mL used for?

What is the recommended positive control for this assay?

A recommended positive control, where one is specified by the manufacturer, is listed on the datasheet or in the assay protocol.

How should this product be stored?

Storage conditions depend on the specific reagent and are listed on the product datasheet, with most antibodies and proteins requiring -20°C unless stated otherwise. It is your responsibility to store the product correctly immediately upon receipt; damage from improper storage after delivery is not eligible for replacement.

Is this product animal-origin-free?

Animal-origin-free status, where applicable, is noted on the datasheet under formulation details.