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Product details
Catalog number BNC743156-100
Price for BNC743156-100
Size / count 1x 100 µL
The specificity of this monoclonal antibody to its intended target was validated by HuProt™ Array, containing more than 19, 000, full-length human proteins. TP53/3156R binds to the C-terminus (aa370-378) of both wild type and mutated p53. When microinjected into nuclei, TP53/3156R blocked re-entry into the S-phase of the cell cycle. Mutation and/or allelic loss of p53 is one of the causes of a variety of mesenchymal and epithelial tumors. If it occurs in the germ line, such tumors run in families. p53 Binds to a DNA consensus sequence, the p53 response element, and it regulates normal cell growth cycle events by activating transcription of genes, involved either in progression through the cycle, or causing arrest in G1 when the genome is damaged. In most transformed and tumor cells the concentration of p53 is increased 51000 fold over the minute concentrations (1000 molecules cell) in normal cells, principally due to the increased half-life (4 h) compared to that of the wild-type (20 min) . p53 Localizes in the nucleus, but is detectable at the plasma membrane during mitosis and when certain mutations modulate cytoplasmic/nuclear distribution. p53 Is the most commonly mutated gene in spontaneously occurring human cancers. Mutations arise with an average frequency of 70% but incidence varies from zero in carcinoid lung tumors to 97% in primary melanomas. High concentrations of p53 protein are transiently expressed in human epidermis and superficial dermal fibroblasts following mild ultraviolet irradiation. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
The catalog number for P53 Tumor Suppressor Protein (TP53/3156R), CF740 conjugate, 0.1mg/mL is BNC743156-100.
P53 Tumor Suppressor Protein (TP53/3156R), CF740 conjugate, 0.1mg/mL is available in the following size(s): 1x 100 µL.
P53 Tumor Suppressor Protein (TP53/3156R), CF740 conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.
P53 Tumor Suppressor Protein (TP53/3156R), CF740 conjugate, 0.1mg/mL is manufactured by Biotium.
Estimated delivery time for P53 Tumor Suppressor Protein (TP53/3156R), CF740 conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.
The safety data sheet for P53 Tumor Suppressor Protein (TP53/3156R), CF740 conjugate, 0.1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.
The specificity of this monoclonal antibody to its intended target was validated by HuProt™ Array, containing more than 19, 000, full-length human proteins. TP53/3156R binds to the C-terminus (aa370-378) of both wild type and mutated p53. When microinjected into nuclei, TP53/3156R blocked re-
Lead times depend on the product type: custom polyclonal antibodies typically take 12-14 weeks, monoclonal antibodies 15-17 weeks, made-to-order recombinant proteins 4-8 weeks, and made-to-order recombinant antibodies 6-8 weeks. These are estimates and may shift due to production delays.
A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.
Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.
