Microphthalmia Transcription Factor (MITF) (MITF/2987R), CF740 conjugate, 0.1mg/mL

Catalog number BNC742987-500

Price for BNC742987-500

Size / count 1x 500 µL

About this product

MITF (microphthalmia transcription factor) is a basic helix-loop-helix-leucine-zipper (bHLH-Zip) transcription factor that regulates the development and survival of melanocytes and retinal pigment epithelium, and also is involved in transcription of pigmentation enzyme genes such as tyrosinase TRP1 and TRP2. MITF has been shown to be phosphorylated by MAP kinase in response to c-kit activation, resulting in upregulation of MITF transcriptional activity. Mutations of the MITF gene are associated with the autosomal dominant hereditary deafness and pigmentation condition, Waardenburg Syndrome type 2A. Multiple isoforms of MITF exist, including MITF-A, MITF-B, MITF-C, MITF-H, and MITF-M, which differ in the amino-terminal domain and in their expression patterns. The MITF-M isoform is restricted to the melanocyte cell lineage. This MAb recognizes a nuclear protein, which is expressed in the majority of primary and metastatic epithelioid malignant melanomas as well as in normal melanocytes, benign nevi and dysplastic nevi. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNC742987-500
Size / count: 1x 500 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: BHLHE32; Class E basic helix-loop-helix protein 32 (bHLHe32) ; CMM8; Mi; Microphthalmia-associated transcription factor; MITF; WS2; WS2A
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: MITF
Gene ID: 4286
NCBI Gene ID: 166017 & 618266
UniProt: O75030
Swiss Prot: O75030
Host: Rabbit
Species Reactivity: Dog, Human
Reactivity: Microphthalmia Transcription Factor
Immunogen: Recombinant full-length human MiTF protein
Target Antigen: Microphthalmia Transcription Factor
Clonality: Recombinant Monoclonal
Clone: MITF/2987R
Type: Primary
Source: Animal
Origin: Animal
Applications: IHC, FFPE (verified)
Positive Control: Jurkat, A-431, HeLa or 501 Mel human melanoma cells. Melanoma.
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% rBSA, 0.05% azide
Molecular Weight: 52-56 kDa (doublet)
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Notes: Protect from light
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG
Species: Oryctolagus cuniculus (domestic rabbit), Bos taurus (bovine)
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: CF740

Frequently Asked Questions

What is the catalog number for Microphthalmia Transcription Factor (MITF) (MITF/2987R), CF740 conjugate, 0.1mg/mL?

The catalog number for Microphthalmia Transcription Factor (MITF) (MITF/2987R), CF740 conjugate, 0.1mg/mL is BNC742987-500.

What sizes are available for Microphthalmia Transcription Factor (MITF) (MITF/2987R), CF740 conjugate, 0.1mg/mL?

Microphthalmia Transcription Factor (MITF) (MITF/2987R), CF740 conjugate, 0.1mg/mL is available in the following size(s): 1x 500 µL.

Is Microphthalmia Transcription Factor (MITF) (MITF/2987R), CF740 conjugate, 0.1mg/mL in stock?

Microphthalmia Transcription Factor (MITF) (MITF/2987R), CF740 conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Microphthalmia Transcription Factor (MITF) (MITF/2987R), CF740 conjugate, 0.1mg/mL?

Microphthalmia Transcription Factor (MITF) (MITF/2987R), CF740 conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for Microphthalmia Transcription Factor (MITF) (MITF/2987R), CF740 conjugate, 0.1mg/mL?

Estimated delivery time for Microphthalmia Transcription Factor (MITF) (MITF/2987R), CF740 conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Microphthalmia Transcription Factor (MITF) (MITF/2987R), CF740 conjugate, 0.1mg/mL?

The safety data sheet for Microphthalmia Transcription Factor (MITF) (MITF/2987R), CF740 conjugate, 0.1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is Microphthalmia Transcription Factor (MITF) (MITF/2987R), CF740 conjugate, 0.1mg/mL used for?

How many freeze-thaw cycles can this product tolerate?

We recommend aliquoting the product upon receipt to avoid repeated freeze-thaw cycles, which can reduce activity and stability. This is your responsibility once the product has been delivered.

Is antigen retrieval required for IHC with this antibody?

Recommended antigen retrieval methods, where applicable, are listed on the datasheet under the IHC protocol section.

What temperature does this product ship at?

Shipping temperature is chosen based on the product’s stability needs, ranging from ambient to dry ice, and is indicated on the product page. Any related shipping fee is shown clearly in your quotation.