- Home
- Shop
- New Categories
- New Blog
- Contact Us
- Cart
- Login
- Register
Select the image to view it full size.
Product details
Catalog number BNC742978-500
Price for BNC742978-500
Size / count 1x 500 µL
Recognizes a protein of 55-62 kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
The catalog number for Cyclin B1 (G2- & M-phase Cyclin) (CCNB1/2978R), CF740 conjugate, 0.1mg/mL is BNC742978-500.
Cyclin B1 (G2- & M-phase Cyclin) (CCNB1/2978R), CF740 conjugate, 0.1mg/mL is available in the following size(s): 1x 500 µL.
Cyclin B1 (G2- & M-phase Cyclin) (CCNB1/2978R), CF740 conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.
Cyclin B1 (G2- & M-phase Cyclin) (CCNB1/2978R), CF740 conjugate, 0.1mg/mL is manufactured by Biotium.
Estimated delivery time for Cyclin B1 (G2- & M-phase Cyclin) (CCNB1/2978R), CF740 conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.
The safety data sheet for Cyclin B1 (G2- & M-phase Cyclin) (CCNB1/2978R), CF740 conjugate, 0.1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.
Recognizes a protein of 55-62 kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accum
A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.
Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.
Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.
