Maltose Binding Protein / MBP-probe (R29.6), CF740 conjugate, 0.1mg/mL

About this product

Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage; such expression vectors frequently encode hybrid fusion proteins consisting in part of prokaryotic and in part, eukaryotic specified proteins. One such system utilizes maltose binding protein (MBP), the 370 amino acid product of the E. coli mal E gene. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be Purified in a one step procedure by affinity chromatography crosslinked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. Expression systems utilizing the MBP fusion tag include pCG-806fx and pMal vectors. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC742847-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

ECK4026; JW3994; MalE; malJ; Maltodextrin binding protein; Maltose ABC transporter periplasmic protein; Maltose binding periplasmic protein; Periplasmic maltose binding protein

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

Not Known

Gene ID

Not Known

NCBI Gene ID

Not Known

UniProt

Not Known

Swiss Prot

Not Known

Host

Mouse

Reactivity

Maltose Binding Protein|MBP-probe

Immunogen

MOS maltose binding protein fusion protein.

Target Antigen

Maltose Binding Protein | MBP-probe

Clonality

Monoclonal

Clone

R29.6

Type

Primary

Source

Animal

Origin

Animal

Applications

WB (verified)

Positive Control

Saos-2 cells. MBP fusion proteins.

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% rBSA, 0.05% azide

Molecular Weight

40 kDa

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Species

Mus musculus (mouse), Bos taurus (bovine)

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF740