Spectrin beta III (SPTBN2) (SPTBN2/2894R), CF405S conjugate, 0.1mg/mL

About this product

Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II), four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin alpha and spectrin beta are present in erythrocytes, whereas spectrin alpha II (also designated fodrin alpha) and spectrin beta I (also designated fodrin beta) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function. Spectrin beta III is highly homologous to both spectrin beta I and spectrin beta II. Spectrin beta III is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta. Spectrin beta 3 is primarily expressed in nervous tissues with highest expression levels in the cerebellum, where it is found in Purkinje cell soma and dendrites.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC042894-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

Beta III spectrin; SCA5; Spectrin beta chain brain 2; Spectrin beta non-erythrocytic 2; Spectrin non-erythroid beta chain 2; Spinocerebellar ataxia 5; SPTBN2

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

SPTBN2

Gene ID

6712

NCBI Gene ID

26915

UniProt

O15020

Swiss Prot

O15020

Host

Rabbit

Species Reactivity

Human

Reactivity

Spectrin Beta-III

Immunogen

Recombinant human SPTBN2 fragment (aa356-475) (exact sequence is proprietary)

Target Antigen

Spectrin Beta-III

Clonality

Recombinant Monoclonal

Clone

SPTBN2/2894R

Type

Primary

Source

Animal

Origin

Animal

Applications

Flow, intracellular (verified) |IHC, FFPE (verified) |WB (verified)

Field of Research

Cytoskeleton

Positive Control

HeLa cells. Pancreas or Liver.

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

246 kDa

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG

Species

Oryctolagus cuniculus (domestic rabbit), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF405S