Rb1 (Tumor Suppressor Protein) (RB1/2313R), CF405S conjugate, 0.1mg/mL

About this product

This antibody recognizes a 105 kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. Its epitope is localized between aa 703-772. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC042313-100

Size / count

1x 100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

OSRC; Osteosarcoma; p105-Rb; PP105; pp110; pRb; Prepro retinoblastoma associated protein; RB1; Retinoblastoma 1; Retinoblastoma-associated protein

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

RB1

Gene ID

5925

NCBI Gene ID

408528

UniProt

P06400

Swiss Prot

P06400

Host

Rabbit

Species Reactivity

Human, Mouse

Reactivity

Rb1

Immunogen

Recombinant full-length human Rb1 protein

Target Antigen

Rb1

Clonality

Recombinant Monoclonal

Clone

RB1/2313R

Type

Primary

Source

Animal

Origin

Animal

Applications

IHC, FFPE (verified)

Field of Research

Tumor suppressors

Positive Control

HeLa, A431 or BT-20 cells. Breast or Colon carcinoma.

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

105 kDa

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG

Species

Oryctolagus cuniculus (domestic rabbit), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF405S