PU.1 (SPI-1) (B-Cell Marker) (PU1/2146), CF488A conjugate, 0.1mg/mL — image 1 of 7

Product details

PU.1 (SPI-1) (B-Cell Marker) (PU1/2146), CF488A conjugate, 0.1mg/mL

Catalog number BNC882146-500

Price for BNC882146-500

Size / count 1x 500 µL

About this product

PU.1 is a member of the ETS family of transcription factors and is important for normal B-cell development. It is expressed in the myeloid lineage and in immature as well as mature B-lymphocytes, with the exception of plasma cells. PU.1 is expressed in germinal center B-cells and mantle B-cells. Various lymphomas are also positive for this marker. It is essential during early B-cell differentiation. The absence of PU.1 results in total block of B-cell development at the pre-pro stage. PU.1 plays a key role in normal myeloid differentiation, and regulates the expression of immunoglobulin and other genes that are important for B-cell development. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNC882146-500
Size / count: 1x 500 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: Transcription Factor spi1; 31kDa Transforming Protein; Hematopoietic Transcription Factor PU.1; SFPI1; SPI1; SPIA; Spleen focus forming virus (SFFV) proviral integration oncogene spi1
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: SPI1
Gene ID: 6688
NCBI Gene ID: 502511
UniProt: P17947
Swiss Prot: P17947
Host: Mouse
Species Reactivity: Human
Reactivity: PU.1
Immunogen: Recombinant fragment (around aa 16-170) of human PU.1 protein (Exact sequence is proprietary)
Target Antigen: PU.1
Clonality: Monoclonal
Clone: PU1/2146
Type: Primary
Source: Animal
Origin: Animal
Applications: Flow, intracellular (verified) |IF (verified) |IHC, FFPE (verified) |WB (verified)
Field of Research: Immunology, Transcription factors
Positive Control: K-562 Cells, THP-1 cells. Lymph Node, Spleen, Hodgkin's Lymphoma, Colon Carcinoma.
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: 40 kDa
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Notes: Protect from light
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG2b, kappa
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: CF488A

Frequently Asked Questions

What is the catalog number for PU.1 (SPI-1) (B-Cell Marker) (PU1/2146), CF488A conjugate, 0.1mg/mL?

The catalog number for PU.1 (SPI-1) (B-Cell Marker) (PU1/2146), CF488A conjugate, 0.1mg/mL is BNC882146-500.

What sizes are available for PU.1 (SPI-1) (B-Cell Marker) (PU1/2146), CF488A conjugate, 0.1mg/mL?

PU.1 (SPI-1) (B-Cell Marker) (PU1/2146), CF488A conjugate, 0.1mg/mL is available in the following size(s): 1x 500 µL.

Is PU.1 (SPI-1) (B-Cell Marker) (PU1/2146), CF488A conjugate, 0.1mg/mL in stock?

PU.1 (SPI-1) (B-Cell Marker) (PU1/2146), CF488A conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures PU.1 (SPI-1) (B-Cell Marker) (PU1/2146), CF488A conjugate, 0.1mg/mL?

PU.1 (SPI-1) (B-Cell Marker) (PU1/2146), CF488A conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for PU.1 (SPI-1) (B-Cell Marker) (PU1/2146), CF488A conjugate, 0.1mg/mL?

Estimated delivery time for PU.1 (SPI-1) (B-Cell Marker) (PU1/2146), CF488A conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for PU.1 (SPI-1) (B-Cell Marker) (PU1/2146), CF488A conjugate, 0.1mg/mL?

The safety data sheet for PU.1 (SPI-1) (B-Cell Marker) (PU1/2146), CF488A conjugate, 0.1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is PU.1 (SPI-1) (B-Cell Marker) (PU1/2146), CF488A conjugate, 0.1mg/mL used for?

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

Can this product be used in multiplex assays?

Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.

What happens if this product doesn’t perform as expected in my experiment?

Send us the catalogue number, product name, lot number, and a summary of your results. Our technical support team will investigate and, where needed, involve the manufacturer. This is covered under our guarantee provided the product was used before its expiry date and stored correctly.