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Product details

HSP27 (Heat Shock Protein 27) (rHSPB1/774), CF568 conjugate, 0.1mg/mL

Catalog number BNC682308-100

Price for BNC682308-100

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Size / count 1x 100 µL

About this product

MAb rHSPB1/774 reacts specifically with heat shock protein HSP27 in human and monkey tissues and cell lines such as MCF-7. HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as by estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSPs and mammalian alpha-crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore HSP27 may be useful in classifying various hormone sensitive tumorsPrimary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number
BNC682308-100
Size / count
1x 100 µL
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9007-83-4
Synonyms
Heat shock 27kDa protein, Estrogen-regulated 24kDa protein, Heat shock 25kDa protein 1, Heat shock 28kDa protein 1, Heat shock protein beta-1, HMN2B, Hsp25, Hsp28, HspB1, Stress-responsive protein 27 (SRP27)
UNSPSC
41116161
UNSPSC Description
Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name
HSPB1
Gene ID
3315
NCBI Gene ID
520973
UniProt
P04792
Swiss Prot
P04792
Host
Mouse
Species Reactivity
Chicken, Chimpanzee, Human, Monkey, Mouse, Rat, Sheep
Reactivity
HSP27
Immunogen
Recombinant full-length human HSPB1 protein
Target Antigen
HSP27
Clonality
Recombinant Monoclonal
Clone
RHSPB1/774
Type
Primary
Source
Animal
Origin
Animal
Field of Research
Endocrinology, Heat shock proteins
Positive Control
~50% of Breast Carcinomas are positive for hsp27 especially those that are also positive for ER and/or PgR.
Stability
2 years
Concentration
0.1 mg/mL
Buffer
PBS, 0.1% BSA, 0.05% azide
Molecular Weight
27 kDa
References & Citations
Edwards DP et. al. Biochem Biophys Research Commun, 93:804-812, 1980. |Ciocca DR et. al. Breast Cancer Research and Treatment, 20:33-42, 199
Shipping Conditions
RT (ND)
Storage Conditions
Stable at room temperature or 37°C (98°F) for 7 days.
Notes
Protect from light
Shelf Life
Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment
IgG1, kappa
Species
Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification
0.05% sodium azide (CAS 26628-22-8)
Conjugate
CF568

Frequently Asked Questions

What is the catalog number for HSP27 (Heat Shock Protein 27) (rHSPB1/774), CF568 conjugate, 0.1mg/mL?

The catalog number for HSP27 (Heat Shock Protein 27) (rHSPB1/774), CF568 conjugate, 0.1mg/mL is BNC682308-100.

What sizes are available for HSP27 (Heat Shock Protein 27) (rHSPB1/774), CF568 conjugate, 0.1mg/mL?

HSP27 (Heat Shock Protein 27) (rHSPB1/774), CF568 conjugate, 0.1mg/mL is available in the following size(s): 1x 100 µL.

Is HSP27 (Heat Shock Protein 27) (rHSPB1/774), CF568 conjugate, 0.1mg/mL in stock?

HSP27 (Heat Shock Protein 27) (rHSPB1/774), CF568 conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures HSP27 (Heat Shock Protein 27) (rHSPB1/774), CF568 conjugate, 0.1mg/mL?

HSP27 (Heat Shock Protein 27) (rHSPB1/774), CF568 conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for HSP27 (Heat Shock Protein 27) (rHSPB1/774), CF568 conjugate, 0.1mg/mL?

Estimated delivery time for HSP27 (Heat Shock Protein 27) (rHSPB1/774), CF568 conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for HSP27 (Heat Shock Protein 27) (rHSPB1/774), CF568 conjugate, 0.1mg/mL?

The safety data sheet for HSP27 (Heat Shock Protein 27) (rHSPB1/774), CF568 conjugate, 0.1mg/mL is available here: https://cdn.gentaur.com/products/37/68dd029c061270361e102343/msds/bnc682308-100.pdf.

What is HSP27 (Heat Shock Protein 27) (rHSPB1/774), CF568 conjugate, 0.1mg/mL used for?

MAb rHSPB1/774 reacts specifically with heat shock protein HSP27 in human and monkey tissues and cell lines such as MCF-7. HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins produced by all organisms studied. HSP27 synthesis is induce

What expression system was used for this recombinant protein?

The expression system, such as E. coli, HEK293, or insect cells, is listed on the datasheet under production details, which can affect post-translational modifications relevant to your application.

Can I return this product if I ordered the wrong one?

As a consumer, you have a 14-day right of withdrawal from receipt under EU consumer protection law, submitted via our withdrawal/cancellation request form. Note this applies to unused, undamaged products, and doesn’t override the expiry/storage conditions on performance-based claims.

Is this product tested for endotoxin levels?

Endotoxin testing status is noted on the datasheet where applicable, particularly for recombinant proteins intended for cell-based or functional assays. If this isn’t listed and you need confirmation, contact support with the catalogue number.

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