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Product details

PU.1 (SPI-1) (B-Cell Marker) (PU1/2146), CF568 conjugate, 0.1mg/mL

Catalog number BNC682146-100

Price for BNC682146-100

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Size / count 1x 100 µL

About this product

PU.1 is a member of the ETS family of transcription factors and is important for normal B-cell development. It is expressed in the myeloid lineage and in immature as well as mature B-lymphocytes, with the exception of plasma cells. PU.1 is expressed in germinal center B-cells and mantle B-cells. Various lymphomas are also positive for this marker. It is essential during early B-cell differentiation. The absence of PU.1 results in total block of B-cell development at the pre-pro stage. PU.1 plays a key role in normal myeloid differentiation, and regulates the expression of immunoglobulin and other genes that are important for B-cell development. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number
BNC682146-100
Size / count
1x 100 µL
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9007-83-4
Synonyms
Transcription Factor spi1; 31kDa Transforming Protein; Hematopoietic Transcription Factor PU.1; SFPI1; SPI1; SPIA; Spleen focus forming virus (SFFV) proviral integration oncogene spi1
UNSPSC
41116161
UNSPSC Description
Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name
SPI1
Gene ID
6688
NCBI Gene ID
502511
UniProt
P17947
Swiss Prot
P17947
Host
Mouse
Species Reactivity
Human
Reactivity
PU.1
Immunogen
Recombinant fragment (around aa 16-170) of human PU.1 protein (Exact sequence is proprietary)
Target Antigen
PU.1
Clonality
Monoclonal
Clone
PU1/2146
Type
Primary
Source
Animal
Origin
Animal
Applications
Flow, intracellular (verified) |IF (verified) |IHC, FFPE (verified) |WB (verified)
Field of Research
Immunology, Transcription factors
Positive Control
K-562 Cells, THP-1 cells. Lymph Node, Spleen, Hodgkin's Lymphoma, Colon Carcinoma.
Stability
2 years
Concentration
0.1 mg/mL
Buffer
PBS, 0.1% BSA, 0.05% azide
Molecular Weight
40 kDa
Shipping Conditions
RT (ND)
Storage Conditions
Stable at room temperature or 37°C (98°F) for 7 days.
Notes
Protect from light
Shelf Life
Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment
IgG2b, kappa
Species
Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification
0.05% sodium azide (CAS 26628-22-8)
Conjugate
CF568

Frequently Asked Questions

What is the catalog number for PU.1 (SPI-1) (B-Cell Marker) (PU1/2146), CF568 conjugate, 0.1mg/mL?

The catalog number for PU.1 (SPI-1) (B-Cell Marker) (PU1/2146), CF568 conjugate, 0.1mg/mL is BNC682146-100.

What sizes are available for PU.1 (SPI-1) (B-Cell Marker) (PU1/2146), CF568 conjugate, 0.1mg/mL?

PU.1 (SPI-1) (B-Cell Marker) (PU1/2146), CF568 conjugate, 0.1mg/mL is available in the following size(s): 1x 100 µL.

Is PU.1 (SPI-1) (B-Cell Marker) (PU1/2146), CF568 conjugate, 0.1mg/mL in stock?

PU.1 (SPI-1) (B-Cell Marker) (PU1/2146), CF568 conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures PU.1 (SPI-1) (B-Cell Marker) (PU1/2146), CF568 conjugate, 0.1mg/mL?

PU.1 (SPI-1) (B-Cell Marker) (PU1/2146), CF568 conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for PU.1 (SPI-1) (B-Cell Marker) (PU1/2146), CF568 conjugate, 0.1mg/mL?

Estimated delivery time for PU.1 (SPI-1) (B-Cell Marker) (PU1/2146), CF568 conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for PU.1 (SPI-1) (B-Cell Marker) (PU1/2146), CF568 conjugate, 0.1mg/mL?

The safety data sheet for PU.1 (SPI-1) (B-Cell Marker) (PU1/2146), CF568 conjugate, 0.1mg/mL is available here: https://cdn.gentaur.com/products/37/68dd02aa061270361e107225/msds/bnc682146-100.pdf.

What is PU.1 (SPI-1) (B-Cell Marker) (PU1/2146), CF568 conjugate, 0.1mg/mL used for?

PU.1 is a member of the ETS family of transcription factors and is important for normal B-cell development. It is expressed in the myeloid lineage and in immature as well as mature B-lymphocytes, with the exception of plasma cells. PU.1 is expressed in germinal center B-cells and mantle B-cells. Var

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.

What should I check when this product first arrives?

Inspect the package immediately for transportation damage, ice loss, leakage, or breakage. Report any issues right away with photos and your order reference; our specialists will determine whether the product is still usable or needs replacing.

Does this cell line or biologic require a Material Transfer Agreement (MTA)?

Some products, including certain cell lines, plasmids, and tissue cultures, are subject to intellectual property terms and may require an MTA before we can ship. We’ll inform you in advance if this product requires one, and additional handling fees may apply.

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