FOXA1 / HNF3A (FOXA1/2230R), CF640R conjugate, 0.1mg/mL

About this product

The transcription factor Forkhead-box A1 (FOXA1), also known as hepatocyte nuclear factor 3-alpha, is a member of the FOX class of transcription factors. HNF-1 (alpha and beta), HNF-3 (alpha, beta and gamma), HNF-4 (alpha and gamma), and HNF-6 compose, in part, a homeoprotein family designated the hepatocyte nuclear factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. FOXA1 is expressed in normal breast ductal epithelium and other epithelium in different organs, such as lung, pancreas, bladder, prostate, and colon. Recently, FOXA1 has been shown to be a major determinant of estrogen-ER activity and endocrine response in breast cancer cells. FOXA1 expression correlates with estrogen receptor (ER) -positivity, especially in luminal subtype A breast cancers, which is associated with favorable prognosis. FOXA1 is useful in the sub-classification of breast carcinomas.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC402230-100

Size / count

1x 100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

Forkhead box protein A1 (FOXA1) ; Hepatocyte nuclear factor 3-alpha (HNF-3-alpha or HNF-3A or HNF3A) ; Transcription factor 3A (TCF-3A or TCF3A)

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

FOXA1

Gene ID

3169

NCBI Gene ID

163484

UniProt

P55317

Swiss Prot

P55317

Host

Rabbit

Species Reactivity

Human, Rat

Reactivity

FOXA1|HNF3A

Immunogen

Recombinant human FOXA1 protein fragment (around aa372-472) (exact sequence is proprietary)

Target Antigen

FOXA1 | HNF3A

Clonality

Recombinant Monoclonal

Clone

FOXA1/2230R

Type

Primary

Source

Animal

Origin

Animal

Applications

Flow, intracellular (verified) |IF (verified) |IHC, FFPE (verified) |WB (verified)

Field of Research

Cancer, Developmental biology, Endocrinology, Transcription factors

Positive Control

HepG-2 or MCF-7 cells. Liver, Pancreas, Bladder, Prostate, Colon and Lung and Breast.

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

49 kDa

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG

Species

Oryctolagus cuniculus (domestic rabbit), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF640R