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Product details

HisSep Ni-NTA Agarose Resin

Catalog number 20502ES10

Discontinued

Price for 20502ES10

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Size / count 10 mL

This variant is discontinued and cannot be added to your cart.

About this product

HisSep Ni-NTA Agarose Resin is based on a cross-linked 6% agarose gel matrix, chemically coupled with tetradentate nitrilotriacetic acid (NTA) ligands. Upon chelation with nickel ions (Ni2+), it forms a highly stable octahedral structure, with nickel ions situated at the center of the octahedron. This structure effectively shields nickel ions from attack by small molecules, making it more stable compared to Ni-IDA resin. It can withstand harsh conditions including certain concentrations of reducing agents, denaturants, or coupling agents. It has become an indispensable resin for purifying His-tagged proteins in laboratory experiments.

Specifications

Catalog number
20502ES10
Size / count
10 mL
Dry ice
No
Hazardous
No
Discontinued
Yes
Field of Research
Protein Research
Shipping Conditions
Blue Ice

Frequently Asked Questions

What is the catalog number for HisSep Ni-NTA Agarose Resin?

The catalog number for HisSep Ni-NTA Agarose Resin is 20502ES10.

Is HisSep Ni-NTA Agarose Resin in stock?

HisSep Ni-NTA Agarose Resin is currently made to order / not held in immediate stock. Contact us for lead time.

What is HisSep Ni-NTA Agarose Resin used for?

HisSep Ni-NTA Agarose Resin is based on a cross-linked 6% agarose gel matrix, chemically coupled with tetradentate nitrilotriacetic acid (NTA) ligands. Upon chelation with nickel ions (Ni2+), it forms a highly stable octahedral structure, with nickel ions situated at the center of the octahedron. Th

What is the purity of this recombinant protein?

Purity, typically assessed by SDS-PAGE, is listed on the datasheet under quality control data.

What do I do if this product arrives with reduced ice or a damaged cold-chain package?

Report this to us immediately with photos and your order reference. Our specialists will assess whether the product is still usable given the cold-chain interruption, and will arrange a replacement or credit if it isn’t.

What host species was used to raise this antibody?

The host species used in production is listed under product specifications on the datasheet, and is relevant when selecting a compatible secondary antibody for your assay.

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