GAD1 / GAD67 (GABAergic Neuronal Marker) (GAD1/2563), CF647 conjugate, 0.1mg/mL

About this product

This MAb recognizes a protein of 67 kDa, which is identified as glutamic acid decarboxylase 1 (GDA1) . There are two forms of glutamic acid decarboxylases (GADs) that are found in the brain: GAD65 (also known as GAD2) and GAD67 (also known as GAD1. GAD65 and GAD67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate-limiting step in the production of GABA (ÃŽ3-aminobutyric acid) from L-glutamic acid. Although both GAD s are found in the brain, GAD65 localizes to synaptic vesicle membranes in nerve terminals, while GAD67 is distributed throughout the cell. GAD67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD65 will transiently activate to assist in GABA production. The loss of GAD65 is detrimental and can impair GABA neurotransmission, however the loss of GAD67 is lethal. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC472563-100

Size / count

1x 100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

67kDa glutamic acid decarboxylase; CPSQ1; DCE1; GAD67; GAD1; Glutamate decarboxylase 1; SCP

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

GAD1

Gene ID

2571

NCBI Gene ID

420036

UniProt

Q99259

Swiss Prot

Q99259

Host

Mouse

Species Reactivity

Human

Reactivity

GAD1|GAD67

Immunogen

Recombinant human GAD1 (GAD67) protein fragment (around aa 72-135) (exact sequence is proprietary)

Target Antigen

GAD1 | GAD67

Clonality

Monoclonal

Clone

GAD1/2563

Type

Primary

Source

Animal

Origin

Animal

Applications

Flow, intracellular (verified)

Field of Research

Neuroscience

Positive Control

K-562 or HEK293 Cells. Pancreas

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

67 kDa

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG2b, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF647