Ubiquitin (Autophagy Marker) (UBB/2122), CF647 conjugate, 0.1mg/mL

About this product

Ubiquitin is a highly conserved and plays an essential role in the ubiquitin-proteasome pathway. In ubiquitination process, it is first activated by forming a thiol-ester complex with the activation component E1, which is then transferred to ubiquitin-carrier protein E2, followed by to ubiquitin ligase E3 for final delivery to epsilon-NH2 of the target protein lysine residue. IkB, p53, cdc25A, Bcl-2 etc. are shown as targets of ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis. Moreover, ubiquitin have been reported to bind covalently with pathological inclusions which are resistant to degradation e.g. neurofibrillary tangles/paired helical filaments in Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease etc.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC472122-100

Size / count

1x 100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

Epididymis secretory protein Li 50; Polyubiquitin B; RPS27A; UBA52; UBA80; UBB; UBC; UBCEP1; UBCEP2; Ubiquitin; Ubiquitin B

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

UBB

Gene ID

7314

NCBI Gene ID

356190 and 520348

UniProt

P0CG47; P0CG48; P62979; P62987 and P62988

Swiss Prot

P0CG47; P0CG48; P62979; P62987 and P62988

Host

Mouse

Species Reactivity

Human

Reactivity

Ubiquitin

Immunogen

Recombinant fragment of human Ubiquitin protein (around aa 1-119) (exact sequence is proprietary)

Target Antigen

Ubiquitin

Clonality

Monoclonal

Clone

UBB/2122

Type

Primary

Source

Animal

Origin

Animal

Applications

IHC, FFPE (verified) |WB (verified)

Field of Research

Ubiquitination

Positive Control

HeLa or Jurkat cells. Alzheimer s Brain

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

9 kDa

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF647