PD-L1 / PDCD1LG1 / CD274 / B7-H1 (Cancer Immunotherapy Target) (PDL1/2746), CF647 conjugate, 0.1mg/mL

About this product

PD-L1 (also known as Programmed Death Ligand 1, CD274, and B7-H1) is one of two cell surface ligands that engage the negative regulatory T cell surface receptor PD-1. PD-L1 is a Type I transmembrane protein expressed on the plasma membrane of cells, such as activated macrophages and some tumor cells. Because binding of PD-1 by PD-L1 has a suppressive effect on the immune system, inhibition of the PD-1/PD-L1 pathway is a major strategy of immune-oncology therapies. PD-L1 is upregulated in certain cancers, and blocking PD-1 or PD-L1 can inhibit cancer growth. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC472746-100

Size / count

1x 100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

B7 homolog 1; B7-H1; CD274; PD-L1; PDCD1 ligand 1; PDCD1L1; PDCD1LG1; Programmed cell death 1 ligand 1

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

CD274

Gene ID

29126

NCBI Gene ID

521989

UniProt

Q9NZQ7

Swiss Prot

Q9NZQ7

Host

Mouse

Species Reactivity

Human, Mouse

Reactivity

B7-H1|CD274|PD-L1|PDCD1LG1

Immunogen

Recombinant fragment of human CD274 protein (around aa 39-191) (exact sequence is proprietary)

Target Antigen

B7-H1 | CD274 | PD-L1 | PDCD1LG1

Clonality

Monoclonal

Clone

PDL1/2746

Type

Primary

Source

Animal

Origin

Animal

Applications

Flow, surface (verified) |IF (verified) |IHC, FFPE (verified) |WB (verified)

Field of Research

Apoptosis, Cancer, Cancer immunotherapy/immune checkpoint

Positive Control

Heart, Placenta, Spleen or Tonsil tissue. RAW, HEK293 or HepG2 cell lysates. Jurkat Cells.

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

37-50 kDa

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG2b, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF647