Nrf2 Recombinant Rabbit Monoclonal Antibody [JE30-14]

About this product

Nuclear factor erythroid 2-related factor 2 (NRF2), also known as nuclear factor erythroid-derived 2-like 2, is a transcription factor that in humans is encoded by the NFE2L2 gene. NRF2 is a basic leucine zipper (bZIP) protein that may regulate the expression of antioxidant proteins that protect against oxidative damage triggered by injury and inflammation, according to preliminary research.[6] In vitro, NRF2 binds to antioxidant response elements (AREs) in the promoter regions of genes encoding cytoprotective proteins. NRF2 induces the expression of heme oxygenase 1 in vitro leading to an increase in phase II enzymes. NRF2 also inhibits the NLRP3 inflammasome. NRF2 appears to participate in a complex regulatory network and performs a pleiotropic role in the regulation of metabolism, inflammation, autophagy, proteostasis, mitochondrial physiology, and immune responses.

Specifications

Catalog number

HA721432

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

erythroid derived 2 antibody HEBP1 antibody like 2 antibody NF E2 related factor 2 antibody NF-E2-related factor 2 antibody NF2L2_HUMAN antibody NFE2 related factor 2 antibody NFE2-related factor 2 antibody Nfe2l2 antibody Nrf 2 antibody NRF2 antibody Nuclear factor (erythroid derived 2) like 2 antibody Nuclear factor antibody nuclear factor erythroid 2 like 2 antibody Nuclear factor erythroid 2 related factor 2 antibody Nuclear factor erythroid 2-related factor 2 antibody Nuclear factor erythroid derived 2 like 2 antibody

Abbreviation

NF-E2-related factor 2, NFE2-related factor 2, NFE2L2

Swiss Prot

Q16236 Human, Q60795 Mouse

Cellular Locus

Cytoplasm, Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Synthetic peptide within Human Nrf2 aa 550-605.

Isotype

IgG

Clone

JE30-14

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell

Positive Control

HeLa whole cell lysate, HeLa treated with 2μM MG-132 for 18 hours whole cell lysate, HCT 116 whole cell lysate, HCT 116 treated with 25μM MG-132 for 4 hours whole cell lysate, NIH/3T3 treated with 10μM MG-132 for 8 hours cell lysate, HCT 116.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IF-Cell: 1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 68 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes