YY1 Monoclonal Antibody

Specifications

Catalog number

AN005360L-01

Size / count

25 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

YY1 is a widely distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. This protein is involved in inhibiting and activating a variety of promoters. YY1 can directly introduce histone deacetylase and histone acetyltransferase into the promoter, thereby activating or inhibiting the promoter, so that histone modification can act on YY1. Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. Binds to the consensus sequence 5'-CCGCCATNTT-3'; some genes have been shown to contain a longer binding motif allowing enhanced binding; the initial CG dinucleotide can be methylated greatly reducing the binding affinity. The effect on transcription regulation is depending upon the context in which it binds and diverse mechanisms of action include direct activation or repression, indirect activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformational DNA changes. Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression.

Abbreviation

YY1

UniProt

P25490

Host

Mouse

Reactivity

Human; Mouse; Rat

Immunogen

Recombinant human YY1 protein expressed by E.coli

Target

YY1, Transcriptional repressor protein YY1, Delta transcription factor, INO80 complex subunit S, NF-E1, Yin and yang 1 (YY-1), INO80S

Clonality

Monoclonal

Clone

4C7

Conjugation

Unconjugated

Applications

WB

Field of Research

Epigenetics and Nuclear Signaling; Neuroscience; Isotype; Loading Controls

Purification

Protein A/G Purification

Concentration

1 mg/mL

Dilution

WB 1:1000-1:2000

Buffer

PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH 7.4

Shipping Conditions

The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light.

Calculated Molecular Weight

45 kDa

Observed Molecular Weight

65 kDa

Isotype

IgG2b