Recombinant Human STAT3 Protein (GST Tag)

Specifications

Catalog number

PDEH100640-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors. Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed:17344214) . May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6) -responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1 (PubMed:17344214) . Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation (By similarity) . May play an apoptotic role by transctivating BIRC5 expression under LEP activation (PubMed:18242580) . Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity.

Abbreviation

STAT3

Synonyms

STAT; Signal Transducer and Activator of Transcription; ADMIO; ADMIO1; APRF; HIES; STAT3; Acute-Phase Response Factor; Signal Transducer and Activator of Transcription 3;1110034C02Rik; Acute Phase Response Factor; AW109958; DNA binding protein APRF; FLJ20882; MGC16063; Signal transducer and activator of transcription 3 (acute phase response factor) ; STAT 3

UniProt

P40763

Accession Number

P40763

Expression System

E.coli

Tag

N-GST

Sequence

Ala2-Ser219

Field of Research

Signal Transduction; Epigenetics and Nuclear Signaling; Stem Cells; Cancer; Cardiovascular; Developmental Biology

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method

Purity

> 85% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

49.8 kDa

Observed Molecular Weight

50 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human