Aly Recombinant Rabbit Monoclonal Antibody [JE36-45]

About this product

Aly/REF export factor, also known as THO complex subunit 4 is a protein that in humans is encoded by the ALYREF gene. The ALYREF gene encodes Aly/REF export factor (ALY; THO complex subunit 4, Tho4; RNA and export factor binding protein 1, Refbp1), a ubiquitously expressed nuclear protein that functions as a molecular chaperone and export adapter involved in nuclear export of spliced and unspliced mRNA. The TRanscription-EXport (TREX) complex, a key player in mRNA export, includes the THO subcomplex, the RNA helicase UAP56, and the RNA-binding protein ALY. In yeast, TREX is recruited co-transcriptionally; in human cells it is recruited during a late step of splicing. The human TREX complex is recruited to a region near the 5' end of mRNA by interaction of ALY and THO with the nuclear cap-binding complex. As a chaperone, ALY promotes dimerization of transcription factors containing basic leucine zipper (bZIP) domains., thereby promoting transcriptional activation. ALY has key roles in 3'-end processing of polyadenylated mRNAs and in nuclear export of both polyadenylated and non-polyadenylated mRNAs. After mRNA binds to ALY, it is apparently transferred to the NXF1-NXT1 heterodimer for export (TAP/NFX1 pathway). The full-length ALY protein (Refbp1-I, 255 amino acids encoded by six exons[8]) has a conserved RNA recognition motif (RRM; amino acids 105-182) flanked by alanine/arginine/glycine-rich sequences; an N-terminal region (amino acids 16-37) is sufficient for RNA binding and interaction with the NXF1-NXT1 heterodimer.

Specifications

Catalog number

HA721961

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Ally of AML-1 and LEF-1 antibody Ally of AML1 and LEF1 antibody ALY antibody ALY/REF antibody Aly/REF export factor antibody BEF antibody bZIP enhancing factor antibody bZIP-enhancing factor BEF antibody REF antibody THO complex 4 antibody THO complex subunit 4 antibody Tho4 antibody thoc4 antibody THOC4_HUMAN antibody Transcriptional coactivator Aly/REF antibody Transcriptional coactivator antibody

Abbreviation

ALYREF, ALY, BEF, THOC4

Swiss Prot

Q86V81 Human, O08583 Mouse,

Cellular Locus

Nucleus, Nucleus speckle, Cytoplasm.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human Aly aa 208-257 / 257.

Isotype

IgG

Clone

JE36-45

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IHC-P, FC

Positive Control

HepG2 cell lysate, K-562 cell lysate, 293T cell lysate, A549 cell lysate, SK-OV-3 cell lysate, HL-60 cell lysate, NIH/3T3 cell lysate, Neuro-2a cell lysate, C6 cell lysate, mouse testis tissue lysate, mouse spleen tissue lysate, rat testis tissue lysate, HepG2, human brain tissue, mouse brain tissue, rat brain tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IF-Cell: 1:250 ;IHC-P: 1:2,000 ;FC: 1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 27 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes