CARD11 Recombinant Rabbit Monoclonal Antibody [JE37-28]

About this product

The protein encoded by this gene belongs to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying a characteristic caspase-associated recruitment domain (CARD). CARD11 (CARMA1) has a domain structure similar to that of CARD10 (CARMA3) and CARD14 (CARMA2) as a member of the CARD-CC family with a C terminal MAGUK domain (the so-called CARMA proteins). The CARD domain of proteins in the CARD-CC family have been shown to specifically interact with BCL10, a protein known to function as a positive regulator of NF-κB activation by recruitment and activation of MALT1. When overexpressed in cells, this protein family activates NF-κB and induces the phosphorylation of BCL10. CARD11 is critical for T cell and B cell function and is activated after T cell receptor or B cell receptor stimulation. After receptor stimulation, CARD11 is phosphorylated by PKC-θ (in T cells) or PKC-β (in B cells). The phosphorylation induces formation of filamentous CARD11 multimers that recruit BCL10 and MALT1, which in turn activates NF-κB. Loss of function mutations in CARD11 cause severe combined immunodeficiency (SCID) since the function of cells critical for adaptive immunity are disrupted.

Specifications

Catalog number

HA722309

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

0610008L17Rik antibody 2410011D02Rik antibody Bcl10 interacting maguk protein 3 antibody BIMP 3 antibody BIMP3 antibody CAR11_HUMAN antibody CARD 11 antibody CARD containing MAGUK protein 3 antibody Card maguk protein 1 antibody CARD-containing MAGUK protein 1 antibody CARD11 antibody CARD11 protein antibody Carma 1 antibody CARMA1 antibody Caspase recruitment domain containing protein 11 antibody Caspase recruitment domain family member 11 antibody Caspase recruitment domain-containing protein 11 antibody MGC133069 antibody

Abbreviation

CARD11, CARMA1

Swiss Prot

Q9BXL7 Human, Q8CIS0 Mouse,

Cellular Locus

Cytoplasm, Membrane raft.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human Carma 1/CARD11 aa 1100-1154.

Isotype

IgG

Clone

JE37-28

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P

Positive Control

K-562 cell lysate, HDLM-2 cell lysate, Raji cell lysate, mouse thymus tissue lysate, mouse spleen tissue lysate, rat thymus tissue lysate, human lymph node tissue, human spleen tissue, mouse thymus tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:2,000 ;IHC-P: 1:200-1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 133 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes